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· D, MIA-PaCa-2 cells were transfected with nonsense or MIF siRNA for 48 h. Conditioned medium from confluent MIA-PaCa-2 cells was mixed 1:1 with fresh medium, pretreated with isotype control mAb, anti-MIF mAb, or 100 μmol/L final concentration of the small-molecule inhibitor ISO-1, and used to replace media from nonsense- and MIF-transfected cells.
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References for MIA PaCa-2 cells: 1. Hussein, D. and Mo, H. d-delta-Tocotrienol-mediated suppression of the proliferation of human PANC-1, MIA PaCa-2, and BxPC-3 pancreatic carcinoma cells. Pancreas, 38: e124-136, 2009. 2.
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